Extensions of MADM (Mosaic Analysis with Double Markers) in Mice
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چکیده
منابع مشابه
Extensions of MADM (Mosaic Analysis with Double Markers) in Mice
Mosaic Analysis with Double Markers (MADM) is a method for generating genetically mosaic mice, in which sibling mutant and wild-type cells are labeled with different fluorescent markers. It is a powerful tool that enables analysis of gene function at the single cell level in vivo. It requires transgenic cassettes to be located between the centromere and the mutation in the gene of interest on t...
متن کاملMosaic analysis with double markers (MADM) in mice.
The human brain comprises more than 100 billion neurons, each of which has an elaborate shape and a complex pattern of connections. To untangle this complexity, it is often useful to visualize one neuron at a time. Mosaic analysis with double markers (MADM) is a genetic method for labeling and manipulating individual neurons. This method was developed in mice and it allows simultaneous labeling...
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The initiation and progression of many human cancers involve either somatic activation of protooncogenes or inactivation of tumor-suppressor genes (TSGs) in sporadic cells. Although sporadic gain-of-function of protooncogenes has been successfully modeled in mice [e.g., Johnson L, Mercer K, Greenbaum D, Bronson RT, Crowley D, Tuveson DA, Jacks T (2001) Nature 410:1111-1116], generating a simila...
متن کاملMosaic Analysis with Double Markers in Mice
We describe a method termed MADM (mosaic analysis with double markers) in mice that allows simultaneous labeling and gene knockout in clones of somatic cells or isolated single cells in vivo. Two reciprocally chimeric genes, each containing the N terminus of one marker and the C terminus of the other marker interrupted by a loxP-containing intron, are knocked in at identical locations on homolo...
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functions. However, the mechanisms of GABAergic neurogenesis in the midbrain are not well understood. Previously, it has been shown that Ascl1 is required for development of GABAergic neurons in the midbrain. Here we have characterized in more detail the role of Ascl1 in the development of distinct subpopulations of midbrain GABAergic neurons. In Ascl1-null mutants GABAergic neurons are complet...
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ژورنال
عنوان ژورنال: PLoS ONE
سال: 2012
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0033332